Identification of Candida species in neonatal septicaemia
Keywords:Brain heart infusion, Potassium hydroxide, Low birth weight, Neonatal intensive care units
Background: Candidemia refers to presence of candida species in blood. It is significant cause of mortality and morbidity in neonatal intensive care unit. Candidiasis is a primary or secondary fungal infection involving a member of genus candida. The aim and objectives of the study was to identify and separate candida isolates obtained from cases of neonatal candidemia and clinical manifestations associated with it and to identify the species other than candida albicans from cases of neonatal candidemia and clinical manifestations associated with them.
Methods: The present study was conducted in the Department of pediatrics, Subharti Medical College from August 2014 to August 2015. Neonates with signs of septicemia were screened and blood culture was done for candida. Candida species were isolated from blood and urine. Blood was inoculated and candida species were identified by Bact/alert method of sampling. Urine was examined by direct microscopy in 10% KOH mount stain.
Results: Out of 527 clinically suspected cases of septicemia screened, 69.3% cases were blood culture positive. Non-Albicans Candida (NAC) were isolated from blood in 86.4% cases of neonatal candidemia compared to candida albicans in 13.6%
Conclusions: The Present study has given us a clear insight into the changing trend of candida species with emergence of non-albicans candida (NAC) species as an important cause of neonatal candidemia from Meerut city: definitely a changing trend.
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