DOI: http://dx.doi.org/10.18203/2349-3291.ijcp20172680

A study on bacteriological profile, drug sensitivity and resistance pattern of isolated organism in neonatal septicaemia in neonatal intensive care unit

Himanshu A. Joshi, Seema S. Shah

Abstract


Background: Neonatal Sepsis is one of the major cause of mortality and morbidity in Neonates. Bacterial pathogens and drug resistance varies from different hospitals areas, regions and countries. Advances in early diagnosis and treatment have led to better prognosis of newborns in NICU. This study will provide the bacterial pathogen causing neonatal sepsis along with their antibiogram. The pattern of susceptibility to antibiotics in NICU at GMERS Medical College centre, was very helpful to start the empirical therapy.

Methods: This retrospective study was carried out in GMERS Medical College, Gandhinagar from February 2015 to January 2016. Period in 228 neonates. Out of which 131 cases were positive for blood culture. The positive blood culture was detected by Bactec blood culture system.

Results: In present study 131 out of 228 cases were culture positive (57.48%). In gram negative organism Klebsiella pneumoniae was the commonest followed by E. coli (3.05%) and Pseudomonas (2.3%) CONS was the most common isolates in from the group. Klebsiella pneumoniae was sensitive to levofloxacin (9816), imipenem (88.15%) and piperacillin+tazobactam (88.15%). This organism was having less sensitivity to routine 1st line antibiotics like ampicillin (9.2%), gentamycin (27.4%), amikeine (35.5%) amoxy+clavulinic (21%) acid, cefotaxime (25%), gram positive CONS having sensitivity to vanceomycin (8.8%), amkacine, ampicillin, levofloxacin, cefotaxime were having limited effect on CONS organism.

Conclusions: There is an increasing trend of antibiotic resistance to the commonly used first line drugs. The pattern of sensitivity is changing hence continuous survelliance for antibiotic susceptibility is needed to ensure correct empirical therapy before blood culture reports are available. 


Keywords


Bacterial Sepsis, Neonates, Resistance, Sensitivity

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References


Gomaa HH, Udo EE, Rajaram U. Neonatal septicemia in Al-Jahra Hospital, Kuwait: etiologic agents and antibiotic sensitivity patterns. Med Princi Pract. 2001;10(3):145-50.

Vergnano S, Sharland M, Kazembe P, Mwansambo C, Heath PT. Neonatal sepsis: an international perspective. Archives of Disease in Childhood-Fetal and Neonatal Edition. 2005;90(3):F220-F224.

Thaver D, Zaidi AK. Burden of neonatal infections in developing countries: a review of evidence from community-based studies. Pediatr Infect Dis J. 2009;28(1):S3-9.

Paolucci M, Landini MP, Sambri V. How can the microbiologist help in diagnosing neonatal sepsis?. Int J Pediatr. 2012:120-39.

Khan SN, Joseph S. Neonatal sepsis: antibiotic sensitivity and resistance pattern of commonly isolated pathogens in a neonatal intensive care unit of a teritiary care hospital, South India. Int J Pharm Bio Sci. 2012;3(4):802-9.

Aurangzeb B, Hameed A. Neonatal sepsis in hospital-born babies: bacterial isolates and antibiotic susceptibility patterns. Journal of the College of Physicians and Surgeons-Pakistan: JCPSP. 2003;11:629-32.

Shaw CK, Shaw P, Thapalial A. Neonatal sepsis bacterial isolates and antibiotic susceptibility patterns at a NICU in a tertiary care hospital in western Nepal: a retrospective analysis. Kathmandu Univ Med J. 2007;5:153-60.

Monsef A, Eghbalian F. Antibiotic sensitivity pattern of common bacterial pathogens in NICU and neonatal ward in Hamedan province of Iran. Health. 2010;2(06):625-9.

Movahedian AH, Moniri R, Mosayebi ZI. Bacterial culture of neonatal sepsis. Iranian J Pub Health. 2006;35(4):84-9

Desai KJ, Malek SS. Neonatal septicemia- bacterial isolates and their antibiotic susciptibitlity pattern. Nati J Integr Res Med. 2010;1(3):12-5.

Anwar SK. Neonatal sepsis- an epideniolagic study. J Pak Med Assoc. 2000;50:91-4.

Mahmood A. Neonatal sepsis, high antibiotic resistance of the Bacterial Pathogens in a NICU in Karachi. J Pak Med Assoc. 2002;52:348-50.