Identification of the bacterial isolates in neonatal septicaemia and their antimicrobial susceptibility in a tertiary care hospital in Uttarakhand, India: a retrospective study

Neerul Pandita; Sanober Wasim; Novneet Kumar Bhat; Vipan Chandra; Barnalli Kakati

doi:10.18203/2349-3291.ijcp20160159

Authors

Neerul Pandita Department of Pediatrics, SRHU, Jolly Grant, Doiwala, Dehradun, Uttarakhand, India
Sanober Wasim Department of Pediatrics, SRHU, Jolly Grant, Doiwala, Dehradun, Uttarakhand, India
Novneet Kumar Bhat Department of Pediatrics, SRHU, Jolly Grant, Doiwala, Dehradun, Uttarakhand, India
Vipan Chandra Department of Pediatrics, SRHU, Jolly Grant, Doiwala, Dehradun, Uttarakhand, India
Barnalli Kakati Department of Microbiology, SRHU, Jolly Grant, Doiwala, Dehradun, Uttarakhand, India

DOI:

https://doi.org/10.18203/2349-3291.ijcp20160159

Keywords:

Septicaemia, Neonate, Resistance, Antibiotics, Drugs

Abstract

Background: Neonatal sepsis refers to systemic bacterial infection documented by positive blood culture in the first four weeks of life and is one of the four leading causes of neonatal mortality and morbidity in India. This study was a retrospective study to identify the bacterial isolates in neonatal septicaemia and their antimicrobial susceptibility in a tertiary care hospital in Uttarakhand, India from January 2013 to June 2015.

Methods: Blood culture in newborns with clinical sign of septicaemia was retrospectively studied. Antimicrobial susceptibility testing was performed by Kirbuay -bauer disc diffusion susceptibility method in accordance to clinical laboratory standard institute guidelines (CLSI).

Results: 10.3% (124 /1200) cases of septicaemia could be confirmed by blood culture. Of these Gram negative could be isolated in (62%) of cases and (38%) were of gram positive isolates. Kliebsiella was the predominant pathogen (42.8%) among the gram negative pathogens followed by E coli (18.1%). CONS (57.4%) was the predominant gram positive pathogen. Of the Gram positives isolates 6% were methicillin resistant S.aureus (MRSA). Polymyxin (77.8%) and tigecycline (72%) were most effective drugs against gram negative isolates were as gram positive organisms showed maximum sensitivity to vancomycin (90%) and linezolid (87%).

Conclusions: This study highlights the growing resistance to commonly used antibiotics; ampicillin, penicillin, ceftriaxone and ceftazidime and also highlights the importance of kleibsiella and Staphylococcus aureus as principal organism responsible for neonatal sepsis in tertiary care settings.

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