Serologic and molecular characterization of rotavirus from children with acute gastroenteritis in Chennai, Tamil Nadu, India
DOI:
https://doi.org/10.18203/2349-3291.ijcp20203167Keywords:
Diarrhea, MDG-IV, Rota virus gastro enteritis, RT-PCR, VP4, VP7Abstract
Background: Rotavirus gastroenteritis is the leading cause of diarrhea in infants and young children worldwide. Although, Rotavirus vaccine has been introduced in 2017 in states like Tamil Nadu, there are reports of the role of Rotavirus as one of high disease burden agents with genetic variants arising, especially from low-income countries like India.
Methods: Authors evaluated stool samples from 507 children with acute gastroenteritis Rotavirus A among the hospitalized children (>5 years) to provide baseline information on changing profile in this state. The stool samples were collected and screened for Rotaviral Antigen by Enzyme Immuno Assay and use of semi-multiplex RT PCR technique was conceded out in order to conclude the P and G genotypes of human rotavirus in rotavirus-positive samples from January 2014 to December 2016 in and around Chennai, India.
Results: Of 507 samples collected 213 (42.01%) were positive for rotavirus antigen by Enzyme Immuno Assay (EIA). The maximum positivity (75%) was in the age group of one to two years. Rotavirus positives were subjected to further VP7 and VP4 molecular characterization and the predominant genotypes identified were G9P[4] followed by G9P[8], G1P[8], G3P[8], G2P[4] and mixed types of G2G9 with P[4] and G4P[6][11] with few untypable strains.
Conclusions: This study had demonstrated the Rota Virus Gastro Enteritis (RVGE) is a common disease affecting the pediatric population and G9P[4], G9P[8] circulating types among the gastroenteritis cases reported in the city and its suburban area. This study in comparison to previous ones shows that the dominant serotypes and circulating genotypes changes from time to time within country. The results have reemphasized the need of rotavirus vaccines with broad serotype coverage which may help in decreasing the disease burden in this region of the country.
References
Szajewska H, Dziechciarz P. Gastrointestinal infections in the pediatric population. Curr Opin Gastroenterol. 2010;26(1):36-44.
Vidya P, Ponnambalam A, Gunasekeran P, Arunagiri K, Sambasivam M, Krishnasami K. Rota virus infections: prevalence, diagnosis and prevention. J Pediatr Sci. 2015;7.
Rotavirus Classification Working Group. Newly assigned genotypes. Available at: https://kuleuven.be/cev/viralmetagenomics/virus-classification/rwwg. Accessed 2016.
Molbak K, Fischer TK, Mikkelsen CS. The estimation of mortality due to rotavirus infections in sub-Saharan Africa; Vaccine. 2000;15;19:393-5.
Van Man N, Luan LT, Trach DD, Thanh NT, Tu PV, Long NT, et al. Epidemiological profile and burden of rotavirus diarrhea in Vietnam: 5 years of sentinel hospital surveillance, 1998-2003. J Inf Dis. 2005;192(Supplement_1):S127-32.
Phan TG, Khamrin P, Quang TD, Dey SK, Takanashi S, Okitsu S, et al. Detection and genetic characterization of group A rotavirus strains circulating among children with acute gastroenteritis in Japan. J Virol. 2007 May 1;81(9):4645-53.
Saravanan P, Ananthan S, Ananthasubramanian M. Rotavirus infection among infants and young children in Chennai, South India. Indian J Med Microbiol. 2004 Oct 1;22(4):212.
Kumar A, Basu S, Vashishtha V, Choudhury P. Burden of rotavirus diarrhea in under-five Indian children. Indian Pediatr. 2016 Jul 1;53(7):607-17.
Shetty M, Brown TA, Kotian M, Shivananda PG. Viral diarrhoea in a rural coastal region of Karnataka India. J Tropical Pediatr. 1995 Oct 1;41(5):301-3.
Babji S, Arumugam R, Sarvanabhavana A. Multi-center surveillance of rotavirus diarrhea in hospitalized children <5 years of age in India, 2009- 2012. Vaccine. 2009;32:10-2.
Badur M, Latha NM, Kumar PR, Dudala SR, Shaik SA, Kang G, Kumar CN. Prevalence of rotavirus diarrhea among under-5 hospitalized children in a Government Tertiary Hospital, Tirupati. J Dr. NTR University of Health Sci. 2015 Apr 1;4(2):112.
Daga N, Sankar J, Sadullah S, Sankaranarayanan VS, Srinivas S, Devaraj H, Devaraj SN. Prevalence of group A rotavirus genotype G1P [8] in Chennai, South India. Int J Contemp Pediatr. 2016 Oct;3(4):1362.
Chennai Metropolitan Development Authority 2012. Available at: https://en.wikipedia.org/wiki/Chennai_City Accessed on 2018.
India Meteorological Department. Ever recorded maximum temperature, minimum temperature and 24 hours heaviest rainfall up to 2010. India Meteorological Department, Pune; 2014:67.
Chennai Regional Website. Indian meteorological department. Available at: https://www.india.gov.in/website-regional-meteorological-centre-chennai Accessed 2012.
World Health Organization, Geneva, Switzerland. Reading on diarrhea. 1992: Student manual. Available at: https://apps.who.int/iris/handle/10665/40343. Accessed 16 June 2012.
Offit PA, Clark MF. Editr: Mandell GL, Bennett JE, Dolin R. Philadelphia, and PA. Churchill Livingstone. Reoviruses. In Principle and practice of infectious diseases; 2000:1696-1703.
Mangayarkarasi V, Prema A, Gunasekaran P, Babu BS, Kaveri K. A unique human rotavirus (non vaccine) G9P4 genotype infection in a child with gastroenteritis. Indian Pediatr. 2012;49(7):569-71.
Gouvea V, Glass RI, Woods P, Taniguchi K, Clark HF, Forrester B, et al., Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clinic Microbiol. 1990;28:276-82.
Staat MA, Azimi PH, Berke T, Roberts N, Bernstein DI, Ward RL, et al. Clinical presentations of rotavirus infection among hospitalized children. The Pediatr Inf Dis J. 2002 Mar 1;21(3):221-7.
Barnes GL, Uren E, Stevens KB, Bishop RF. Etiology of acute gastroenteritis in hospitalized children in Melbourne, Australia, from April 1980 to March 1993. J Clin Microbiol. 1998;36(1):133-8.
Ford-Jones EL, Wang E, Petric M, Corey P, Moineddin R, Fearon M. Hospitalization for community-acquired, rotavirus-associated diarrhea: a prospective, longitudinal, population-based study during the seasonal outbreak. Arch Pediatr Adolesc Med. 2000;154:578-85.
John BM, Devgan A, Mitra B. Prevalence of rotavirus infection in children below two years presenting with diarrhea. Med J Arm Forces India. 2014 Apr 1;70(2):116-9.
Bass CW, Dorsey KN. Rotavirus and other agents of viral gastroenteritis. In Nelson Textbook of Pediatrics Edited by: Richard E and Behrman F. Raven Press. Philadelphia; 2004:107-110.
Mullick S, Mukherjee A, Ghosh S, Pazhani GP, Sur D, Manna B, et al. Community based case-control study of rotavirus gastroenteritis among young children during 2008-2010 reveals vast genetic diversity and increased prevalence of G9 strains in Kolkata. PloS One. 2014;9(11):e112970.
Gómara MI, Kang G, Mammen A, Jana AK, Abraham M, Desselberger U, et al. Characterization of G10P (11) rotaviruses causing acute gastroenteritis in neonates and infants in Vellore, India. J Clin Microbiol. 2004 Jun 1;42(6):2541-7.
Tamil Nadu to introduce rotavirus vaccine to combat diarrhoea. Available at: www.timesofindia.indiatimes.com/city/chennai/tn-to-introduce to /58424798.cms. Accessed 17 April 2017.
