Study of blood cultures by BACTEC method in pediatric patients of Chhattisgarh, India


  • Meenakshi S. Morey Department of Microbiology, Raipur Institute of Medical Science, Raipur, Maharashtra, India
  • Nirmal Channe Department of Microbiology, Government of Medical Collage, Nagpur, Maharashtra, India



Pediatric Intensive care unit, Micro gram, BACTEC 9050


Background: 356 children aged  between 2 to 4 years admitted at PICU were selected for study B.D BACTEC 9050 system was used for incubation bottle  were incubated until microbial growth was detected, BACTEC 9050 is an automated blood culture system which responds to concentration of  Co2 produced by metabolism microorganism or consumption of O2, needed for growth of microorganism.

Methods: The antibiotic used were Amoxicin 250 mcg, penicillin 100 unit linezoid 50 Mcg, vancomycin 50 mcg, ampicillin 100 mcg, Azithromycin 100 Mcg, pipericillin/ Tozabactum, 100/50mcg, ceforazone/ salbactum 50/30 mcg, cefoxitin 50 mcg, cefepime 50 mcg, Amikacin 50 mcg, Imipenen 30 mcg, ceftazidime- clavulanic acid..

Results: The observed organized were 90 (25.2%) CoNS, 72 (20.2%) Entrobacter, 70 (19.6%) Klebesiella  spp, 40(11.1%) Enterococcus, 22(6.1%) S, auresis, 22 (6.1%) E coli,  20(5.6%) citrobacters, 20(5.6%) Acinitobacter. This blood culture study  by BACTEC 9050 in pediatric patients in few minutes.

Conclusions: BACTEC-9050 method was helpful to treat especially in children because most of diseases in children are idiopathic and life threatening.


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