DOI: http://dx.doi.org/10.18203/2349-3291.ijcp20163678

Prevalence of group A rotavirus genotype G1P[8] in Chennai, South India

Nikita Daga, Jamuna Sankar, Sakeena Sadullah M.S., V. S. Sankaranarayanan, S. Srinivas, H. Devaraj, S. Niranjali Devaraj

Abstract


Background:Rotavirus is one of the significant causes of severe diarrhoea in children less than 5 years of age, worldwide. The objective of this study was to determine the distribution of the G and P genotypes of rotavirus seasonal variation and to monitor if there was any emerging genotype or unusual strain circulating among children with diarrhoea, age less than 5 years, in Chennai, India.

Methods: 171 stool specimens were collected from children with moderate to severe diarrhoea admitted to hospital or outpatient wards from January to December 2013 and information was collected from patients for rotavirus vaccination status.

Results:Out of the 171 stool specimens, 109 specimens were detected positive for rotavirus by using VIKIA rapid kit method. The G and P genotypes were identified by reverse transcription-hemi nested PCR. G1P[8] (63.30%) was the most prevalent strain observed. Other genotypes observed were G1P[4], G1P[6], G2P[4], G9P[4], G9P[8], G12P[6], G12P[8] and some mixed infections were also observed with different rotavirus strains such as G1P[4]P[8] and G1G2P[6].

Conclusions:In this study, out of 109 positive patients, 7 patients who were vaccinated with 2 doses of rotarix vaccine were also positive for different strains of rotavirus. Rotavirus diarrhoea was found to occur throughout the year with a peak in the month of July and mostly in children aged 0-24 months.  


Keywords


Diarrhoea, Genotyping, G1P[8], Rotavirus, Rotarix vaccine

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References


Black RE, Cousens S, Johnson HL, Lawn JE, Rudan I, Bassani DG, Jha P, et al. Global, regional, and national causes of child mortality in 2008: a systematic analysis. Lancet. 2010;375(9730):1969-87.

World Health Organization. Global networks for surveillance of rotavirus gastroenteritis, 2001-2008. Weekly Epidemiology Record. 2008;83:421-8.

Tate JE, Burton AH, Pinto BC. 2008 estimate of worldwide rotavirus-associated mortality in children younger than 5 years before the introduction of universal rotavirus vaccination programmes: a systematic review and meta-analysis. The Lancet Infectious Diseases. 2012;12:136-41.

Estes MK, Cohen J. Rotavirus Gene Structure and Function. Microbiological Reviews. 1989;53:410-49.

Gomara IM, Kang G, Gray J. Rotavirus genotyping: keeping up with an evolving population of human rotaviruses. J Clinic Virology. 2004;31:259-65.

Agocs MM, Serhan F, Yen C. WHO Global rotavirus surveillance network: a strategic review of the first 5 years, 2008-2012. Morbidity and mortality weekly report (MMWR) WHO. 2014;63:613-44.

Parashar UD, Hummelman EG, Bresee JS, Miller MA, Glass RI. Global illness and deaths caused by rotavirus disease in children. Emerging Infect Dis. 2003;9:565-72.

Morris SK, Awasthi S, Khera A. Rotavirus mortality in India: estimates based on a nationally representative survey of diarrhoeal deaths. Bulletin of the World Health Organization. 2012;90:720-7.

World Health Organization. Rotavirus vaccines. Weekly Epidemiology Record. 2013;88:49-64.

Rotavirus disease and vaccines in Africa. Available at https:// www.path.org /publications /files /VAD _rotavirus_africa_fs.pdf. Accessed on 12 July 2016.

Gentsch JR, Glass RI, Woods P. Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clinic Microbio. 1992;30:1365-73.

Gouvea V, Glass RI, Woods P. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clinical Microbio. 1990;28:276-82.

Gomara IM, Green J, Brown DWG, Desselberger U, Gray JJ. Comparison of specific and random priming in the reverse transcriptase polymerase chain reaction for genotyping group A rotaviruses. J Virological Methods. 1999;78:93-103.

Banerjee I, Ramani S, Primrose B. Modification of rotavirus multiplex RT-PCR for the detection of G12 strains based on characterization of emerging G12 rotavirus strains from South India. J Med Virology. 2007;79:1413-21.

Babji S, Arumugam R, Sarvanabhavana A. Multi-center surveillance of rotavirus diarrhea in hospitalized children <5 years of age in India, 2009-2012. Vaccine. 2014;32:10-2.

Babji S, Arumugam R, Peters A, Kang G. Detection and characterization of rotaviruses from children less than 5 years hospitalized with acute gastroenteritis in Nagercoil. Indian J Med Microbiology. 2013;31:69-71.

Rajendran P, Kang G. Molecular epidemiology of rotavirus in children and animals and characterization of an unusual G10P[15] strain associated with bovine diarrhea in south India. Vaccine. 2014;32:89-94.

Namjoshi GS, Mitra M, Lalwani SK. Rotavirus gastroenteritis among children less than 5 years of age in private outpatient setting in urban India. Vaccine. 2014;32:36-44.

Kahn G, Fitzwater S, Tate J. Epidemiology and prospects for prevention of rotavirus disease in India. Indian Pediatrics. 2012;49:467-74.

Kang G, Arora R, Chitamber SD. Multicenter, hospital-based surveillance of rotavirus disease and strains among Indian Children Aged < 5 Years. J Infectious Dis. 2009;200:147-53.

Reesu R, Bhattacharya D, Chaaithanya IK. Emergence of an unusual genotype of rotavirus in Andaman and Nicobar Islands, India. Intervirology. 2013;56:134-9.

Mishra V, Awasthi S, Nag VL, Tandon R. Genomic diversity of group A rotavirus strains in patients aged 1-36 months admitted for acute watery diarrhoea in northern India: a hospital-based study. Clinic Microbio Infection. 2010;16:45-50.

Assis ASF, Valle DA, Antunes GR. Rotavirus epidemiology before and after vaccine introduction. J Pedia. 2013;89:470-6.

Braeckman T, Herck KH, Meyer N. Effectiveness of rotavirus vaccination in prevention of hospital admissions for rotavirus gastroenteritis among young children in Belgium: case-control study. BMJ. 2012;345:4752.

Vesikari T, Karvonen A, Prymula R. Efficacy of human rotavirus vaccine against rotavirus gastroenteritis during the first 2 years of life in European infants: randomized, double-blind controlled study. Lancet. 2007;370:1757-63.

Vesikari T. Rotavirus vaccination: a concise review. Clinic Microbio Infection. 2012;18:57-63.