Mid-stream clean catch urine collection in newborns: a non-invasive and safe technique

Rajendra Kumar, Nithin ., Sudha Rudrappa


Background: The aim of this study is to determine the success rate and safety of a non-invasive technique to obtain clean-catch midstream urine samples in newborns.

Methods: Prospective bedside clinical study. After obtaining written informed consent,120 consecutive newborns admitted in NICU with no dehydration, poor feeding, need for immediate urine sample by invasive method  for whom urine collection was advised for various reasons who met the inclusion criteria were included in the study with consent being taken from the parents. After adequate milk intake supra pubic and lumbar para vertebral areas were stimulated in repeated cycles of 30 s until micturition began.

Results: Success rate in obtaining a midstream urine sample within 5 min. The success rate was 90%. The mean time taken to collect urine was 64.24s, for males it was 62.55s and for females 65.93s.

Conclusions: The technique has been demonstrated to be safe, quick and effective. The discomfort and time consumption usually associated with bag collection methods as well as invasive techniques can be avoided.


Clean-catch, Midstream, Newborns, Urine

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Verrier K, Banerjee J, Boddy SA, et al. National Collaborating Centre for Women’s and Children’s Health. Guideline Development Group. Urinary tract infection in children: diagnosis, treatment and long-term management. Clinical Guideline. 2007:44-8.

Fernández ML, Merino NG, García AT, Seoane BP, de la Serna Martínez M, Abad MT, et al. A new technique for fast and safe collection of urine in newborns. Arch Dis Childhood. 2013;98(1):27-9.

Altuntas N, Tayfur AC, Kocak M, Razi HC, Akkurt S. Midstream clean-catch urine collection in newborns: a randomized controlled study. Europ J Pediatr. 2015;174(5):577-82.

Alam MT, Coulter JB, Pacheco J, Correia JB, Ribeiro MG, Coelho MF, et al. Comparison of urine contamination rates using three different methods of collection: clean-catch, cotton wool pad and urine bag. Annals Trop Paediatr. 2005;25(1):29-34.

Prasad RS, Smith SJ, Wright H. Lower abdominal pressure versus external bladder stimulation to aid bladder emptying in multiple sclerosis: a randomized controlled study. Clin Rehabil. 2003;17:42-7.

Dasgupta P, Haslam C, Goodwin R, Fowler CJ. The ‘Queen Square bladder stimulator’: a device for assisting emptying of the neurogenic bladder. Br J Urol. 1997;80:234-7.

Jansson UB, Hanson M, Hanson E, Hellström AL, Sillén U. Voiding pattern in healthy children 0 to 3 years old: a longitudinal study. J Urol. 2000;164(6):2050-4.

Amir J, Ginzburg M, Straussberg R, Varsano I. The reliability of midstream urine culture from circumcised male infants. Am J Dis Child. 1993;147:969-70.

Saez-Llorens X, Umana MA, Odio CM, Lohr JA. Bacterial contamination rates for non-clean-catch and clean-catch midstream urine collections in uncircumcised boys. J Pediatr. 1989;114(1):93-5.

Davies P, Greenwood R, Benger J. Randomised trial of vibrating bladder stimulator the time to pee study. Arch Dis Child. 2008;93:423-4.