DOI: http://dx.doi.org/10.18203/2349-3291.ijcp20163167

Bacteriological profile and antibiotic sensitivity patterns of blood cultures

K. Ashwin Reddy, S. Uday Kanth

Abstract


Background:Neonatal septicemia is a significant cause of morbidity and mortality worldwide especially so in developing countries. To reduce the mortality caused by neonatal septicemia, it became vital to diagnose it as soon as possible and treat with administration of appropriate antibiotics. The objective of the study was bacteriological spectrum in blood culture of neonates admitted in a hospital, and antibiotic susceptibility pattern of blood culture positive isolates.

Methods: A total of 593 blood culture sample were received from NICU admissions for a period of 15 months were included for this study. Under aseptic precautions, 1 ml of blood was collected from a peripheral vein and inoculated into a bottle of Brain Heart Infusion broth and was incubated for 7 days. Repeated sub-culturing was done as per standard procedures. Inoculation on blood agar and Mac-Conkey’s agar plates were made. Any growth was subjected for identification by appropriate biochemical tests. Antibiotic susceptibility testing was done by disc diffusion method.

Results:Of the 593 cases studied 12.14 % were blood culture positive. Among the blood culture positive neonates 67% were male neonates. Late onset septicemia (87.5%) was more common than early onset septicemia (12.5%). Gram negative organisms 46 (63.88%) were predominant than gram positive organisms 18 (25%). Klebsiella pneumonia 18 (25%), Citrobacter 10 (13.88%), and Pseudomonas auroginosa was found in 7 (9.72%). The other organisms isolated were Escherichia coli 06 (8.33%), Enterobacter 04 (5.55%), Gram positive organisms were obtained in 18 (25.00%) out of 72 cases. MSSA 06 (8.33%), Enterococci 06 (8.33%) was the commonest organisms isolated. Most of the isolates were more susceptible imipenem, meropenem, and ciprofloxacin to amikacin antibiotics.

Conclusions:Blood culture remains the gold standard for the diagnosis of neonatal septicemia. Periodic surveillance of organisms and their antibiotic sensitivity patterns are essential to understand and to prevent emergence of resistant organisms. Effective/Correct selection of antibiotic is essential to decrease mortality and morbidity in the vulnerable group of neonatal population.


Keywords


Blood culture, Klebsiella pneumonia, Neonatal septicaemia

Full Text:

PDF

References


Gotoff SP, Behrman RE. Neonatal septicaemia. J Pediatr. 1970;76(1):142-53.

Aggarwal R, Sarkar N, Deorari AK. Sepsis in the Newborn. Indian J Pediatr. 2001;68(12):1143-7.

Desai KJ, Malek SS, Parikh A. Neonatal Septicemia: Bacterial Isolates & Their Antibiotic Susceptibility Patterns. Gujrat Med J. 2011;66(1):13-5.

Roy I, Jain A, Kumar M. Bacteriology of Neonatal Septicemia in Tertiary care Hospital of Northern India. Indian J Med Microbiol. 2002;20(3):156-9.

National Neonatal Perinatal Database. Report for the year 2002-03. National Neonatology Forum, India.

Takkar VP, Bhakoo ON, Narang A. Scoring system for the Prediction of Early Neonatal Infections. Indian Pediatr. 1974;11(9):597-60.

Paul VK, Singh M. Diagnosis and Treatment of Neonatal Sepsis. Indian Pediatr. 1986;23:1023-35.

Mathur NB. Neonatal Sepsis. Indian Pediatr. 1996;33:663-74.

Tallur SS, Kasturi AV, Nadgir SD. Clinico bacteriological Study of Neonatal Septicemia in Hubli. Indian J Pediatr. 2000;67(3):169-74.

Nawshad Uddin Ahmed ASM, Chowdhury AMAK, Hoque M. Clinical and bacteriological profile of neonatal septicaemia in a tertiary level pediatric hospital in Bangaldesh. Indian Pediatr. 2002;39:1034-9.

Movahedian AH, Moniri R, Mosayebi Z. Bacterial

Culture of Neonatal Sepsis. Iranian J Public Health. 2006;35(4):84-9.

Chacko B, Sohi I. Early Onset Neonatal Sepsis. Indian J Pediatr. 2005;72(1):23-6.

Khatua SP, Das AK, Chatterjee BD. Neonatal septicemia. Indian J Pediatr. 1986;53(4):509-14.

Karthikeyan G, Premkumar K. Neonatal Sepsis: Staphylococcus aureus as the Predominant Pathogen. Indian J Pediatr. 2001;68(8):715-7.

Darmstdt L. Clinical and Bacteriological Profile of Neonatal Septicemia in a Tertiary level Pediatric Hospital in Bangladesh. Indian Peditr. 2002;39:1034-8.

Shrestha P. Clinical and Bacteriological Profile of Blood Culture Positive Sepsis in Newborns. J Nepal Paediatr Society. 2007;27(2):64-7.

Jaswal RS, Kaushl RK, Goel A. Role of C - reactive protein in Deciding Duration of Antibiotic Therapy in Neonatal Septicemia. Indian Pediatr. 2003;40:880-3.