Published: 2020-03-21

Correlation of serum leptin levels with clinical and biochemical parameters in obese children

Lingaraja Gowda C. Patil, Srinivas S.



Background: Leptin plays a crucial role in the regulation of appetite, glucose homeostasis and body fat. Authors described various clinical and biochemical parameters of obese children aged 6 to 18 years visiting to outpatient department in a tertiary care hospital followed by their correlation with serum leptin levels. It was a prospective observational study.

Methods: Obese children with Body Mass Index >2 standard deviation according to WHO chart were included. Endocrinological and syndromic obese children were excluded. Authors first compared leptin level of obese children with age and sex matched nonobese children. Followed by correlation of serum leptin levels with various clinical and laboratory parameters in obese children. Pearson’s correlation coefficient was used to compute the strength of relationship of leptin with various quantitative parameters. Mann-Whitney Test was also used when standard deviation was high, to calculate statistical significance.

Results: Leptin concentration was significantly higher in obese children than non-obese. No significant difference in sex distribution was found. Serum leptin levels showed positive correlation with BMI, skin fold thickness and abdominal circumference, and blood pressure. No statistical correlation of leptin with biochemical parameters such as dyslipidemia, fasting blood glucose and insulin resistance was seen.

Conclusions: In this study though serum leptin levels had a positive correlation with various clinical parameters, but no statistically significant correlation was seen with biochemical parameters. There is need for further investigation with a larger sample size on the role of leptin in childhood obesity.



Body mass index, Dyslipidemia, Insulin resistance, Leptin, Obesity

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Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM. Positional cloning of the mouse obese gene and its human homologue. Nature. 1994 Dec 1;372(6505):425-32.

Klein S, Coppack SW, Mohamed-Ali V, Landt M. Adipose tissue leptin production and plasma leptin kinetics in humans. Diabetes. 1996 Jul 1;45(7):984-7.

Ahima RS, Saper CB, Flier JS, Elmquist JK. Leptin regulation of neuroendocrine systems. Front Neuroendocrinol. 2000 Jul 1;21(3):263-307.

Argente J, Barrios V, Chowen JA, Sinha MK, Considine RV. Leptin plasma levels in healthy Spanish children and adolescents, children with obesity, and adolescents with anorexia nervosa and bulimia nervosa. J Pediatr. 1997 Dec 1;131(6):833-8.

Lahlou N, Landais P, De Boissieu D, Bougneres PF. Circulating leptin in normal children and during the dynamic phase of juvenile obesity: relation to body fatness, energy metabolism, caloric intake, and sexual dimorphism. Diabetes. 1997 Jun 1;46(6):989-93.

Dubey S, Kabra M, Bajpai A, Pandey RM, Hasan M, Gautam RK, et al. Lead Article. Ind Pediatr. 2007;44:257-62.

Considine RV, Sinha MK, Heiman ML, Kriauciunas A, Stephens TW, Nyce MR, et al. Serum immunoreactive-leptin concentrations in normal-weight and obese humans. New Eng J Med. 1996 Feb 1;334(5):292-5.

Reiterer EE, Sudi KM, Mayer A, Limbert-Zinterl C, Stalzer-Brunner C, Füger G, et al. Changes in leptin, insulin, and body composition in obese children during a weight reduction program. J Pediatr Endocrinol Metab. 1999;12(6):853-62.

Lönnqvist F, Arner P, Nordfors L, Schalling M. Overexpression of the obese (ob) gene in adipose tissue of human obese subjects. Nature Ned. 1995 Sep;1(9):950-3.

Montague CT, Farooqi IS, Whitehead JP, Soos MA, Rau H, Wareham NJ, et al. Congenital leptin deficiency is associated with severe early-onset obesity in humans. Nature. 1997 Jun;387(6636):903-8.

Strobel A, Issad T, Camoin L, Ozata M, Strosberg AD. A leptin missense mutation associated with hypogonadism and morbid obesity. Nature Gene. 1998 Mar;18(3):213-5.

Owen GM. Measurement, recording, and assessment of skinfold thickness in childhood and adolescence: report of a small meeting. Am J Clin Nutri. 1982;35:629-38.

Alberty RR, Albertyová D. Serum lipid growth curves for children and adolescents in predicting adult dyslipidemia (Data from the Slovak Lipid Community Study). Advan Biol Chem. 2013 Oct 10;2013.

Association AD. Diagnosis and classification of diabetes mellitus. Diabetes Care 2006; 29 (Suppl 1):S43-8.

Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC, et al. Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentration in man. Diabetol. 1985;28:412-4.

Sudi K, Gallistl S, Tröbinger M, Reiterer Ε, Payerl D, Aigner R, et al. Insulin and insulin resistance index are not independent determinants for the variation in leptin in obese children and adolescents. J Pediatr Endocrinol Metab. 2000;13(7):923-32.

Zimmet P, Hodge A, Nicolson M, Staten M, De Courten M, Moore J, et al. Serum leptin concentration, obesity, and insulin resistance in Western Samoans: cross sectional study. Bmj. 1996 Oct 19;313(7063):965-9.

Considine RV, Sinha MK, Heiman ML, Kriauciunas A, Stephens TW, Nyce MR, et al. Serum immunoreactive-leptin concentrations in normal-weight and obese humans. New Eng J Med. 1996 Feb 1;334(5):292-5.

Ostlund Jr RE, Yang JW, Klein S, Gingerich R. Relation between plasma leptin concentration and body fat, gender, diet, age, and metabolic covariates. J Clin Endocrinol Metab. 1996 Nov 1;81(11):3909-13.

Rao GSN, Gurumurthy P, Gururajan P, Arumugam SB, Kirthivasan SV, Cherian KM, et al. Clinical and Biochemical Parameters in Relation to Serum Leptin Levels in South Indian Children and Adolescents. Ind J Pediatr. 2010;77:555-9.

Nagy TR, Gower BA, Trowbridge CA, Dezenberg C, Shewchuk RM, Goran MI. Effects of gender, ethnicity, body composition, and fat distribution on serum leptin concentrations in children. J Clin Endocrinol Metab. 1997 Jul 1;82(7):2148-52.

Maffei Á, Halaas J, Ravussin E, Pratley RE, Lee GH, Zhang Y, et al. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nature Med. 1995 Nov;1(11):1155-61.

Al-Daghri NM, Al-Attas OS, Al-Rubeaan K, Mohieldin M, Al-Katari M, Jones AF, et al. Serum leptin and its relation to anthropometric measures of obesity in pre-diabetic Saudis. Cardio Diabetol. 2007 Jul 7;6:18.

Minocci A, Savia G, Lucantoni R, Berselli ME, Tagliaferri M, Calo G, et al. Leptin plasma concentrations are dependent on body fat distribution in obese patients. Inter J Obes. 2000 Sep;24(9):1139-44.

Pilcova R, Sulcova J, Hill M, Bláha P, Lisá L. Leptin levels in obese children: effects of gender, weight reduction, and androgens. Physiol Res. 2003 Jan 1;52(1):53-60.

Valle M, Gascon F, Martos R, Bermudo F, Ceballos P, Suanes A. Relationship between high plasma leptin concentrations and metabolic syndrome in obese pre-pubertal children. Inter J Obes. 2003 Jan;27(1):13-8.

Nishina M, Kikuchi T, Yamazaki H, Kameda K, Hiura M, Uchiyama M. Relationship among systolic blood pressure, serum insulin and leptin, and visceral fat accumulation in obese children. Hyperten Res. 2003;26(4):281-8.

Chu NF, Wang DJ, Shieh SM. Obesity, leptin and blood pressure among children in Taiwan: the Taipei Children's Heart Study. Am J Hyperten. 2001 Feb 1;14(2):135-40.

Chu NF, Chang JB, Shieh SM. Plasma leptin, fatty acids, and tumor necrosis factor‐receptor and insulin resistance in children. Obes Res. 2003 Apr;11(4):532-40.

Nobili V, Manco M, Ciampalini P, Diciommo V, Devito R, Piemonte F, et al. Leptin, free leptin index, insulin resistance and liver fibrosis in children with non-alcoholic fatty liver disease. Eur J Endocrinol. 2006 Nov 1;155(5):735-43.

Misra A, Arora N, Mondal S, Pandey RM, Jailkhani B, Peshin S, et al. Relation between plasma leptin and anthropometric and metabolic covariates in lean and obese diabetic and hyperlipidaemic Asian Northern Indian subjects. Diabe, Nutri Metab. 2001 Feb;14(1):18-26.

Tamer L, Ercan B, Unlü A, Sucu N, Pekdemir H, Eskandari G, et al. The relationship between leptin and lipids in atherosclerosis. Ind Heart J. 2002;54(6):692-6.

Franks PW, Brage S, Luan JA, Ekelund U, Rahman M, Farooqi IS, et al. Leptin predicts a worsening of the features of the metabolic syndrome independently of obesity. Obe Res. 2005 Aug;13(8):1476-84.

Hamidi A, Fakhrzadeh H, Moayyeri A, Heshmat R, Ebrahimpour P, Larjani B. Metabolic syndrome and leptin concentrations in obese children. Ind J Pediatr. 2006 Jul 1;73(7):593-6.